Synthesis of fluorescein labeled 7-methylguanosinemonophosphate.
نویسندگان
چکیده
Binding of eIF4E to the cap structure (m(7)GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence-polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.
منابع مشابه
Synthesis and Applicationof New Fluorescein Analogues
In this project, new derivatives of fluorescein were synthesized using the reaction of maleic anhydride and saccharin with phenol derivatives in the presence of ZnCl2, and their structures were elucidated by UV, IR, and NMR spectroscopy. Fluorimetry studies showed that, the synthesized compounds can be utilized as fluorescent agents, and their efficiencies were dependent on pH of solution. Acco...
متن کاملTuning the pK(a) of fluorescein to optimize binding assays.
The phenolic pKa of fluorescein varies depending on its environment. The fluorescence of the dye varies likewise. Accordingly, a change in fluorescence can report on the association of a fluorescein conjugate to another molecule. Here, we demonstrate how to optimize this process with chemical synthesis. The fluorescence of fluorescein-labeled model protein, bovine pancreatic ribonuclease (RNase...
متن کاملSynthesis and primer properties of oligonucleotides containing 3'-deoxypsicothymidine units, labeled with fluorescein at the 1'-position.
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.
متن کاملDesign, Synthesis and Labeling of Tripeptide Derivatives with 99mTc as Hypoxia Imaging Agents
Background: Many diseases, including cancer, ischemia and tumors show degrees of tissue hypoxia.Therefore, it is useful to detect tissue hypoxia with compounds that can penetrate and remain in hypoxic cells in order to diagnose cancer at early stages. Peptides with low molecular weight, susceptible to redox environment, with easier penetration into tissues and low antigenic characteristics are ...
متن کاملScleral permeability of a small, single-stranded oligonucleotide.
Developing more effective ocular drug delivery systems is essential to improving the treatment of posterior segment eye disease. The large target area provided by the sclera and potentially less vision threatening complications are advantages of transscleral administration compared to more traditional modalities of drug delivery to the posterior segment. We aimed to determine the permeability c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 14 10 شماره
صفحات -
تاریخ انتشار 2004